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Caspase-1 Assay Kit (Colorimetric) LS-K5-100

Size: 100 Assays
Price: $620
USA Shipment Only
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Description: Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity. K5AssayResult1.jpg
Caspase- 1 activity in 1 µg uninduced and induced Jurkat cell lysate.
Caspase-1 Assay Kit (Colorimetric)
LS-K5-100
100 Assays (also available as a 25 Assay kits)
Quantitative Assay Kit
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.
Cell and tissue lysates
Colorimetric (400 or 405 nm Absorbance)
1-2 Hours
Mammalian
  • Cell Lysis Buffer
  • 2X Reaction Buffer
  • YVAD-pNA (4 mM)
  • DTT (1 M)
  • Dilution Buffer
Shipped on gel packs, stored at -20°C, 12 month shelf life.
For research use only.
Data Sheet DataSheet    SDS Sheet SDS  

 


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