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Adenosine Deaminase Activity (ADA) Assay Kit (Colorimetric) LS-K2-100

Size: 100 Assays
Price: $550
USA Shipment Only
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Description: Adenosine Deaminase (ADA) (E.C. 3.5.4.4.) is an enzyme that catalyzes the conversion of adenosine and 2’-deoxyadenosine to inosine and 2’-deoxyinosine. Adenosine Deaminase is widely distributed in various tissues and cells. There are two isoforms, ADA1 and ADA2. ADA1 is widely expressed in most cells in the body, particularly in lymphocytes and macrophages. It is present in the cytosol, nucleus and found associated with dipeptidyl peptidase-4 on the cell membrane. ADA2 was first found in the spleen but is predominantly found in the plasma and serum. Increased serum ADA levels are found in certain infectious diseases such as tuberculosis and various liver diseases such as acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis to name a few. Adenosine Deaminase is also a marker for T-lymphocyte proliferation. In LSBio's ADA Activity Assay, inosine formed from the breakdown of adenosine is converted to uric acid with ADA Convertor and ADA Developer. The uric acid is measured at OD 293 nm. The kit measures total Activity of Adenosine Deaminase with limit of quantification of 1 mU recombinant Adenosine Deaminase. K2_AssayResult1.jpg
ADA specific activity in rat brain lysate and Jurkat cell lysate.
Adenosine Deaminase Activity (ADA) Assay Kit (Colorimetric)
ADA Activity Assay Kit (Colorimetric)
LS-K2-100
100 Assays
Quantitative Assay Kit
Detection of Adenosine Deaminase activity
Purified recombinant protein, Nuclear Extract, Cell and tissue lysate
Colorimetric (293 nm Absorbance)
1 Hour
1 mU recombinant Adenosine Deaminase
  • ADA Assay Buffer (10x)
  • ADA Convertor
  • ADA Developer
  • ADA Substrate
  • ADA Positive Control
  • Inosine Standard (10 mM)
  • U.V. transparent plate (96-well)
Shipped on gel packs, stored at -20°C, 12 month shelf life.
For research use only.
Data Sheet DataSheet    SDS Sheet SDS  

 


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