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Acid Phosphatase Activity Assay Kit (Colorimetric) LS-K15-500

Size: 500 Assays
Price: $470
USA Shipment Only
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Description: Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. LSBio’s Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect µU acid phosphatase activity in samples. K15_AssayResult1.jpg
Measurement of Acid Phosphatase activity (40 minute assay).
Acid Phosphatase Activity Assay Kit (Colorimetric)
LS-K15-500
500 Assays
Quantitative Assay Kit
Phosphate concentrations between 1 µM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
Colorimetric (405 nm Absorbance)
Mammalian
  • AP Assay Buffer
  • pNPP Substrate (10 TAB)
  • AP Enzyme
  • Stop Solution
Shipped on gel packs, stored at -20°C, 12 month shelf life.
For research use only.
Data Sheet DataSheet    SDS Sheet SDS  

 


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