Yeast Ubiquitin-like Protein Modifier Hub1

Rabbit

Yeast (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Unmodified

This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

• Western blot (1:500 - 1:2000)
• ELISA (1:1000 - 1:5000)

This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immuno-precipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. A 9.7 kD band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.