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Catalog Number Size Price
LS-C60081-100 100 µg (1.1 mg/ml) $425 
Antibody

Yeast Rad9 Antibody (phospho‑Ser1260) LS‑C60081

Yeast Rad9 Antibody (phospho‑Ser1260) LS‑C60081

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60081-100
100 µg
1.1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Rad9 antibody LS-C60081 is an unconjugated rabbit polyclonal antibody to yeast Yeast Rad9. Validated for ELISA.
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ELISA
Immunogen
Synthetic peptide corresponding to aa 1249-1263 of 1309 of yeast RAD9 protein.
Epitope
pSer1260
Specificity
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. No reactivity is expected against the human analog of RAD9. Reactivity against RAD9 from other sources is unknown
Usage
This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown. Mature RAD9 is reported to have an apparent molecular weight of ~140kD.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Reviews (0)

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Yeast Rad9 Antibody - Anti-Rad9 Antibody- Western Blot. Affinity purified phospho-specific antibody to yeast Rad9 at pS1260 was used at a 1:200 dilution incubated overnight at 4° C to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kD) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Rad53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110 kD bands (asterisk). Detection occurred using a 1:5000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Species: Yeast
Applications: Western blot, ELISA
F9 / Factor IX ELISA Kit
Reactivity: Mouse
Range: 0.16-10 ng/ml

Requested From: United States
Date Requested: 10/20/2019