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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C60075-100 100 µg (1 mg/ml) $425 
Yeast Rad9 Antibody - Affinity purified antibody to yeast Rad9 (1pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Detection occurred using a 1:5,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Yeast Yeast Rad9 Antibody (WB) LS‑C60075

Polyclonal Rabbit anti‑Yeast Yeast Rad9 Antibody (WB) LS‑C60075

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60075-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Rad9 antibody LS-C60075 is an unconjugated rabbit polyclonal antibody to yeast Yeast Rad9. Validated for ELISA and WB.
Target
Yeast Rad9
Synonyms
Rad9
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Non-Reactivity
Human, Mouse
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:100 - 1:500)
  • ELISA (1:5000)
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1120-1145 from the aa1309 yeast Rad9 protein conjugated to KLH.
Specificity
Reacts equally with both phosphorylated and non- phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown.
Usage
This pan reactive polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of the peptide and protein. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown. Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation. This product has been assayed against 0.1 ug of immunizing peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) catalog no. TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5000 is suggested for this product. Reactivity was detected against both the phosphorylated and non-phosphorylated form (S1129 and pS1129) of the immunizing peptide. This antibody appears to be pan reactive for both forms of the protein. Dilute the antibody 1:100 to 1:500 for immunoblotting. Researchers should determine optimal titers for other applications.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 8/12/2020