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Catalog Number Size Price
LS-C60075-100 100 µg (1 mg/ml) $425 
Antibody

Yeast Rad9 Antibody (phospho‑Ser1129) LS‑C60075

Yeast Rad9 Antibody (phospho‑Ser1129) LS‑C60075

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60075-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Rad9 antibody LS-C60075 is an unconjugated rabbit polyclonal antibody to yeast Yeast Rad9. Validated for ELISA and WB.
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA (1:100 - 1:500)
Immunogen
Synthetic peptide corresponding to aa 1125-1139 of 1309 of yeast Rad9 protein conjugated to KLH.
Epitope
pSer1129
Specificity
an internal sequence of yeast Rad9 at the S1129 residue. Antiserum was purified against the immunizing peptide. This pan reactive polyclonal antibody reacts equally with both phosphorylated and nonphosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown
Usage
This pan reactive polyclonal antibody was tested by immunoblotting and ELISA. Data from both immunoblotting and ELISA indicate the antibody is pan reactive with both the phosphorylated and non-phosphorylated forms of the peptide and protein. Immunoblotting detects yeast Rad9 protein. No reactivity is expected against human and mouse homologs. Reactivity to Rad9 from others sources is unknown. Although not tested, this antibody is likely functional by immunohistochemistry and immunoprecipitation. This product has been assayed against 0.1 ug of immunizing peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) catalog no. TMBE-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5000 is suggested for this product. Reactivity was detected against both the phosphorylated and non-phosphorylated form (S1129 and pS1129) of the immunizing peptide. This antibody appears to be pan reactive for both forms of the protein. Dilute the antibody 1:100 to 1:500 for immunoblotting. Researchers should determine optimal titers for other applications.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/16/2019