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Catalog Number Size Price
LS-C17553-100 100 µg $755 
Antibody

Yeast Rad9 Antibody (aa1249‑1263) LS‑C17553

Yeast Rad9 Antibody (aa1249‑1263) LS‑C17553

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$755 
LS-C17553-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Yeast Yeast Rad9
Application:
ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Rad9 antibody LS-C17553 is an unconjugated rabbit polyclonal antibody to yeast Yeast Rad9. Validated for ELISA.
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ELISA (1:6000)
Immunogen
A synthetic peptide corresponding to aa 1249-1263, ksqmkllerlrsqis, of yeast RAD9 protein.
Epitope
aa1249-1263
Specificity
Recognizes yeast RAD9 protein. Mature RAD9 is reported to have an apparent MW:~140kD. No reactivity is expected against the human analogs of RAD9.
Usage
Suitable for use in ELISA. ELISA: 1:6000 -1:60000.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Reviews (0)

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Yeast Rad9 Antibody - Anti-Rad9 Antibody- Western Blot. Affinity purified phospho-specific antibody to yeast Rad9 at pS1260 was used at a 1:200 dilution incubated overnight at 4° C to detect Rad9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa ~60 kD) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 was by treatment with ATP and Rad53 kinase. Rad53 kinase autophosphorylates and appears cross reactive as it is detected on the blot as 90 and 110 kD bands (asterisk). Detection occurred using a 1:5000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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Range: 0.312-20 ng/ml

Requested From: United States
Date Requested: 10/18/2019