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Catalog Number Size Price
LS-C60091-100 100 µg (0.75 mg/ml) $567 
Yeast Mer2 Antibody - Anti-Mer2 pS30 Antibody - Western Blot. Western blot of affinity purified anti-S. cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5000 dilution. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.

Polyclonal Rabbit anti‑Saccharomyces cerevisiae Yeast Mer2 Antibody (WB) LS‑C60091

Polyclonal Rabbit anti‑Saccharomyces cerevisiae Yeast Mer2 Antibody (WB) LS‑C60091

Antibody:
Yeast Mer2 Rabbit anti-Saccharomyces cerevisiae Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Saccharomyces cerevisiae
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60091-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Yeast Mer2 Rabbit anti-Saccharomyces cerevisiae Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Saccharomyces cerevisiae
Format:
Unconjugated, Unmodified

Specifications

Description
Yeast Mer2 antibody LS-C60091 is an unconjugated rabbit polyclonal antibody to saccharomyces cerevisiae Yeast Mer2. Validated for ELISA, IP and WB.
Target
Saccharomyces cerevisiae Yeast Mer2
Host
Rabbit
Reactivity
Saccharomyces cerevisiae (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
Specificity
A BLAST analysis was used to suggest minimal cross reactivity with Mer2 homologues from other sources.
Applications
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:25000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kD in size corresponding to Mer2 protein by western blotting in the appropriate cell lysate or extract. This antibody is reactive with both phosphorylated and unphosphorylated Mer2 at the S30 position.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024