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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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XPO1 / CRM1 Antibody LS‑C661891

CRM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody
Immunohistochemistry - Anti-CRM1 Picoband Antibody
Immunohistochemistry - Anti-CRM1 Picoband Antibody
CRM1 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody
Immunohistochemistry - Anti-CRM1 Picoband Antibody
CRM1 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody
IF analysis of CRM1 using anti-CRM1 antibody CRM1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CRM1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot - Anti-CRM1 Picoband Antibody
Flow Cytometry analysis of SiHa cells using anti-CRM1 antibody. Overlay histogram showing SiHa cells stained with anti-CRM1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U87 cells using anti-CRM1 antibody. Overlay histogram showing U87 cells stained with anti-CRM1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CRM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody
Immunohistochemistry - Anti-CRM1 Picoband Antibody
Immunohistochemistry - Anti-CRM1 Picoband Antibody
CRM1 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody
Immunohistochemistry - Anti-CRM1 Picoband Antibody
CRM1 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- CRM1 Antigen Affinity purified polyclonal antibody
IF analysis of CRM1 using anti-CRM1 antibody CRM1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CRM1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Western blot - Anti-CRM1 Picoband Antibody
Flow Cytometry analysis of SiHa cells using anti-CRM1 antibody. Overlay histogram showing SiHa cells stained with anti-CRM1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U87 cells using anti-CRM1 antibody. Overlay histogram showing U87 cells stained with anti-CRM1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRM1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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XPO1 / CRM1 Antibody LS‑C661891

Antibody:
Rabbit Polyclonal to Human XPO1 / CRM1
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C661891-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human XPO1 / CRM1
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CRM1 antibody LS-C661891 is an unconjugated rabbit polyclonal antibody to human CRM1 (XPO1). Validated for IHC and WB.
Target
Human XPO1 / CRM1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
XPO1 / CRM1 antibody was raised against e.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
Specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. .
Presentation
5mg BSA 0.9mg NaCl 0.2mg Na2HPO4 0.05mg NaN3 per 100ug antibody
Reconstitution
Reconstitute with 0.2ml distilled H2O.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About XPO1 / CRM1
O14980 NM_003400 NP_003391.1

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Requested From: United States
Date Requested: 7/22/2019
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