LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
XPO1 / CRM1 Antibody LS‑C500034
CRM1 antibody LS-C500034 is an unconjugated rabbit polyclonal antibody to CRM1 (XPO1) from human and mouse. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C500034-50
50 µg
$285
LS-C500034-100
100 µg
$385

Popular XPO1 / CRM1 Products

Anti-CRM1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Monkey, Bat, Bovine, Dog, Horse, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Fetal heart cell lysate. Antibody concentration: 0.5 ug/ml. Gel concentration: 6%-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: Western blot
Anti-XPO1 / CRM1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence
Anti-XPO1 / CRM1 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay

Product Description

CRM1 antibody LS-C500034 is an unconjugated rabbit polyclonal antibody to CRM1 (XPO1) from human and mouse. Validated for ELISA, IF, IHC and WB.

Specifications

Target
Human XPO1 / CRM1
Synonyms
XPO1, CRM1, yeast, homolog, Exp1, Exportin-1, CRM1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
XPO1 / CRM1 antibody was raised against recombinant Human Exportin-1 protein (625-845AA)
Specificity
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About XPO1 / CRM1
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. O14980 NM_003400 NP_003391.1


Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;
Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;
Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;
Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic tissue using XPO1 Antibody at dilution of 1:100

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using XPO1 Antibody at dilution of 1:100

Immunofluorescence

Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using XPO1 Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;
Positive WB detected in:K562 whole cell lysate,NIH/3T3 whole cell lysate;All lanes:XPO1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 124 KDa;Observed band size: 124 KDa;

Requested From: United States
Date Requested: 11/16/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy