CRM1 antibody LS-B1776 is an unconjugated rabbit polyclonal antibody to CRM1 (XPO1) from human, bat, bovine and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human, Monkey, Bat, Bovine, Dog, Horse, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Mouse, Rat, Hamster, Rabbit, Zebrafish (at least 90% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 3 µg/ml)
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XPO1 / CRM1 antibody was raised against amino acids 390-408 (ASPLLSGSQHFDVPPRRQL) of human Exportin-1 protein were used as the immunogen. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Chicken, Platypus, Xenopus (100%); Mouse, Rat, Hamster, Rabbit, Opossum, Zebrafish (95%); Stickleback, Pufferfish (89%).
Amino acids 390-408 (ASPLLSGSQHFDVPPRRQL) of human Exportin-1 protein.
Immunohistochemistry: LS-B1776 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1776 was determined to be 10 ug/ml.
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.