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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WWOX Antibody (aa89‑107) LS‑C2942 Best Seller
Note: This antibody replaces LS-C90730, LS-C11082
WWOX antibody LS-C2942 is an unconjugated rabbit polyclonal antibody to WWOX from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C2942-100
100 µl (1 mg/ml)
$335

Popular WWOX Products

Anti-WWOX antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemical analysis of WWOX staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemistry - Anti-WWOX Picoband antibody
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

WWOX antibody LS-C2942 is an unconjugated rabbit polyclonal antibody to WWOX from human, mouse, rat and other species. Validated for IF, IHC, IP and WB.

Specifications

Target
Human WWOX
Synonyms
WWOX, D16S432E, FRA16D, FOR, HHCMA56, PRO0128, SDR41C1, WOX1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Hamster (tested or 100% immunogen sequence identity)
Predicted
Bat, Dog, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC (1:1000)
  • Immunofluorescence (1:1000)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
WWOX antibody was raised against a synthetic peptide corresponding to residues 89-107 [RLAFTVDDNPTKPTTRQRY] of the human protein. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Hamster, Elephant, Bovine (100%); Gibbon, Panda, Bat, Dog, Rabbit, Pig (95%); Monkey, Horse, Opossum (89%).
Epitope
aa89-107
Specificity
WW domain-containing oxidoreductase.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About WWOX
Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm. Q9NZC7 NM_016373 NP_057457.1


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Images

Immunohistochemistry

Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.
Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.

Immunofluorescence

L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.
L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.

Western blot

Detection of WWOX in human breast cancer lysates (MCF-7).
Detection of WWOX in human breast cancer lysates (MCF-7).

Western blot

Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)
Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)

Immunoprecipitation

3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.
3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.

Immunohistochemistry

Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.
Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.

Immunofluorescence

L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.
L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.

Western blot

Detection of WWOX in human breast cancer lysates (MCF-7).
Detection of WWOX in human breast cancer lysates (MCF-7).

Western blot

Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)
Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)

Immunoprecipitation

3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.
3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.

Immunohistochemistry

Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.
Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.

Immunofluorescence

L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.
L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.

Western blot

Detection of WWOX in human breast cancer lysates (MCF-7).
Detection of WWOX in human breast cancer lysates (MCF-7).

Western blot

Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)
Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)

Immunoprecipitation

3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.
3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.

Immunohistochemistry

Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.
Formalin-fixed paraffin-embedded human skin tissue stained using LS-C2942.

Immunofluorescence

L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.
L929 cells stained with both LS-C2942 (WWOX, green) and DAPI (nuclei, blue). Confocal microscopy.

Western blot

Detection of WWOX in human breast cancer lysates (MCF-7).
Detection of WWOX in human breast cancer lysates (MCF-7).

Western blot

Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)
Detection of WWOX in 30 ug of lysate from murine fibroblast L929 cell lysates using LS-C2942. (Duplicate lanes)

Immunoprecipitation

3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.
3 ug of LS-C2942 immunoprecipitated the 46 kD WWOX protein from the cytosol of Molt4 T-cells, treated with nocodazole (10uM, 24 hr.) to synchronize mitosis.

Requested From: United States
Date Requested: 10/15/2018

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