Bovine WC1 Surface Antigen

Mouse

Bovine (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

CC101

FITC

Protein G purified

Unmodified

Con A stimulated bovine lymphocytes.

Recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform. The bovine WC1 cell surface antigen is expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. Immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homolog of WC1, which is a 180 kD molecule. In pigs the 180 kD molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8.

• Flow Cytometry (1:1 - 1:10)

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide, 1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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