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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WC1 / ATP7B Antibody (N‑Terminus) IHC‑plus™ LS‑B2302 Best Seller
Note: This antibody replaces LS-C2764, LS-C74359
ATP7B antibody LS-B2302 is an unconjugated rabbit polyclonal antibody to ATP7B (WC1) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B2302-100
100 µl (1 mg/ml)
$425
Description
ATP7B antibody LS-B2302 is an unconjugated rabbit polyclonal antibody to ATP7B (WC1) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human WC1 / ATP7B
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
WC1 / ATP7B antibody was raised against a synthetic peptide made to an internal sequence near the N-terminus of human ATP7b
Epitope
N-Terminus
Specificity
ATP7b.
Usage
Immunohistochemistry: LS-B2302 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2302 was determined to be 5 ug/ml.
Presentation
Tris-citrate/phosphate buffer, 0.1% sodium azide.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
About WC1 / ATP7B
P35670 NM_000053 NP_000044.2

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ATP7B Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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Anti-ATP7B antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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Anti-WC1 / ATP7B antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Publications (1)

1
Predictive and prognostic value of human copper transporter 1 (hCtr1) in patients with stage III non-small-cell lung cancer receiving first-line platinum-based doublet chemotherapy. Chen HH, Yan JJ, Chen WC, Kuo MT, Lai YH, Lai WW, Liu HS, Su WC. Lung cancer (Amsterdam, Netherlands). 2012 Feb;75:228-34. [Full Text Article] [PubMed:21788094] [PMC:PMC3319119]

Customer Reviews (0)


Images

Immunohistochemistry

Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ATP7B antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ATP7b detected in 20 ug of mouse brain membrane fraction using WC1 / ATP7B Antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/13/2018
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