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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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VIPAS39 Antibody (aa443-493)

VIPAS39 antibody LS-C290197 is an unconjugated rabbit polyclonal antibody to VIPAS39 from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C290197-100
100 µl (1 mg/ml)
$395
Description
VIPAS39 antibody LS-C290197 is an unconjugated rabbit polyclonal antibody to VIPAS39 from human and mouse. Validated for IP and WB.
Target
Human VIPAS39
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Epitope
aa443-493
Specificity
Region between residue 443 and 493 of human VPS33B Interacting Protein, Apical-Basolateral Polarity Regulator using the numbering given in entry NP_071350.2 (GeneID 63894).
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About VIPAS39
Q9H9C1 NM_022067 NP_071350.2

Popular VIPAS39 Products

Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot
Western blot. All lanes: VIPAS39 antibody at 12 ug/ml. Lane 1: HepG2 cells. Lane 2: A431 cells. Lane 3: 293T cells. Lane 4: mouse kidney tissue. Lane 5: HeLa cells. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 57 kDa. Observed band size: 57 kDa.
Species: Human, Mouse
Applications: Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA

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Images

Western blot

Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western blot

Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western blot

Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western blot

Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Detection of Human and Mouse VIPAR by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-VIPAR antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. VIPAR was also immunoprecipitated by rabbit anti-VIPAR antibody BL12390, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Requested From: United States
Date Requested: 1/23/2019
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