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Catalog Number Size Price
LS-C744980-25 25 µl (1.51 mg/ml) $275 
VEGFA / VEGF Antibody - Western blot using the Protein-A Purified anti-bovine VEGF-A antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kDa (arrowhead). Approximately 2 µg of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA diluted 1:10. The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Bovine VEGFA / VEGF Antibody (Liquid, WB) LS‑C744980

Polyclonal Rabbit anti‑Bovine VEGFA / VEGF Antibody (Liquid, WB) LS‑C744980

Antibody:
VEGFA / VEGF Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C744980-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
VEGFA / VEGF Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Liquid

Specifications

Description
VEGF antibody LS-C744980 is an unconjugated rabbit polyclonal antibody to bovine VEGF (VEGFA). Validated for ELISA and WB.
Target
Bovine VEGFA / VEGF
Synonyms
VEGFA | VPF | Vascular permeability factor | VEGF | VEGF-A | MVCD1
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Predicted
Human, Mouse, Rat, Cat, Dog, Guinea pig, Horse, Pig, Sheep, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Liquid
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.
Specificity
This antibody is specific for bovine VEGF-A protein. A BLAST analysis was used to suggest cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against human, horse, dog, cat, or guinea pig based on 95% homology; and to rat and mouse based on 93% homology. Cross-reactivity with VEGF-A from other sources has not been determined.
Applications
  • Western blot (1:333)
  • ELISA
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VEGFA / VEGF
P15692 NM_003376 NP_003367.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021