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Catalog Number Size Price
LS-C745298-25 25 µl (1 mg/ml) $275 
VDAC1 / PORIN Antibody - Western Blot of rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.
VDAC1 / PORIN Antibody - Western Blot of rabbit anti-VDAC/Porin antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: LNCaP Lysate Lane 7: A-172 Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 31 kDa for VDAC\Porin.
VDAC1 / PORIN Antibody - Western Blot of rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 32 kDa, 32 kDa for VDAC/Porin. Other band(s): Not identified
VDAC1 / PORIN Antibody - Western Blot of rabbit Anti-VDAC/Porin Antibody. Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead). Other band(s): none.
VDAC1 / PORIN Antibody - Western Blot of rabbit anti-VDAC/Porin antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: LNCaP Lysate Lane 7: A-172 Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 31 kDa for VDAC\Porin.
VDAC1 / PORIN Antibody - Western Blot of rabbit anti-VDAC/Porin antibody. Lane 1: MW ladder. Lane 2: Mouse Heart WCL. Load: 10 µg per lane. Primary antibody: VDAC/Porin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 32 kDa, 32 kDa for VDAC/Porin. Other band(s): Not identified
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Polyclonal Rabbit anti‑Human VDAC1 / PORIN Antibody (IHC, WB) LS‑C745298

Polyclonal Rabbit anti‑Human VDAC1 / PORIN Antibody (IHC, WB) LS‑C745298

Antibody:
VDAC1 / PORIN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Goat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745298-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
VDAC1 / PORIN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Goat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
PORIN antibody LS-C745298 is an unconjugated rabbit polyclonal antibody to PORIN (VDAC1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human VDAC1 / PORIN
Synonyms
VDAC1 | HVDAC1 | Plasmalemmal porin | Porin 31HM | PORIN | PORIN-31-HL | Vdac5 | VDAC | Mitochondrial porin | Porin 31HL | VDAC-1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Goat, Pig (tested or 100% immunogen sequence identity)
Predicted
Fish, Avian, Mammal, Reptile (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.
Specificity
A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VDAC1 / PORIN
P21796 NM_003374 NP_003365.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/13/2021