Human VCAN / Versican

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Recombinant protein corresponding to aa1344-1554 from human Versican isoform V0, expressed in E. coli (P13611).

aa1344-1554

Recognizes human Versican. Species sequence Homology: For immunizing sequence, aa1344-1554: canine (80%), mouse (60%).

• IHC (10 µg/ml)
• ICC (10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 0.1-0.2 ug/ml. Detection limit for rhVersican is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/ml detects Versican in Hela cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Immunohistochemistry: 10 ug/ml detects Versican in cryosections of E13.5 mouse embryos. Tissue was fixed with PBS containing 4%PFA for 20 min. at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton and 1% BSA for 45 minutes at RT. After blocking, sections were incubated with diluted primary antibody for 3 hours at RT followed by fluorochrome-coupled anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS. Direct ELISA: 0.5-1.0 ug/ml. Detection limit for rhVersican is ~0.1 ng/well. Optimal dilutions should be determined by each laboratory.

Lyophilized from PBS, 5% Trehalose

Reconstitute in sterile PBS.

Store at -20°C to -70°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13611 NCBI: NM_004385 NP_004376.2