Virus V5 Tag

Mouse

Virus (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

SV5-Pk4

Unconjugated

Protein G purified

Unmodified

A recombinant peptide corresponding to the PK tag fused to a class II-restricted T helper cell epitope.

Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. The antibody has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).

• Immunofluorescence
• Western blot (1:100 - 1:500)
• Immunoprecipitation
• ELISA

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.