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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C806416-100 100 µl (1 mg/ml) $325 
LS-C806416-200 200 µl (1 mg/ml) $375 
USP7 / HAUSP Antibody - 1:100 staining human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
USP7 / HAUSP Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
USP7 / HAUSP Antibody - Western blot analysis of HepG2 cells using USP7 antibody. The lane on the left is treated with the antigen-specific peptide.
USP7 / HAUSP Antibody - 1:100 staining human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
USP7 / HAUSP Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
USP7 / HAUSP Antibody - Western blot analysis of HepG2 cells using USP7 antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal USP7 / HAUSP Antibody (IHC, IF, WB) LS‑C806416

Polyclonal USP7 / HAUSP Antibody (IHC, IF, WB) LS‑C806416

Antibody:
Rabbit Polyclonal to Human USP7 / HAUSP
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C806416-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human USP7 / HAUSP
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HAUSP antibody LS-C806416 is an unconjugated rabbit polyclonal antibody to HAUSP (USP7 ) from human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Human USP7 / HAUSP
Synonyms
USP7 | Deubiquitinating enzyme 7 | HAUSP | TEF1 | Ubiquitin specific protease 7 | Ubiquitin thioesterase 7
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Bovine, Dog, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:20 - 1:50)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthesized peptide derived from human USP7.
Specificity
USP7 Antibody detects endogenous levels of total USP7.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20 °C for up to 12 months
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About USP7 / HAUSP
Q93009 NM_003470 NP_003461.2

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Requested From: United States
Date Requested: 2/27/2020