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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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US01 / p115 Antibody LS‑C332251
P115 antibody LS-C332251 is an unconjugated rabbit polyclonal antibody to p115 (US01) from human and mouse. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C332251-50
50 µl
$235
LS-C332251-100
100 µl
$315
LS-C332251-200
200 µl
$425

Popular US01 / p115 Products

Rabbit antibody to VDP. Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0.01M containing Triton X 0.005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to internal region of Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein): IgG diluted 1:100 in the blocking buffer for 30 minutes. Wells were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1:100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot
Anti-USO1 / p115 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-US01 / p115 antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

P115 antibody LS-C332251 is an unconjugated rabbit polyclonal antibody to p115 (US01) from human and mouse. Validated for IF, IHC, IP and WB.

Specifications

Target
Human US01 / p115
Synonyms
USO1, p115, Protein USO1 homolog, TAP, Vesicle docking protein p115, Vesicle-docking protein, US01, USO1 vesicle transport factor, VDP
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
US01 / p115 antibody was raised against a synthetic peptide of human USO1.
Specificity
Human US01 / p115
Usage
The predicted MW is 107kDa/109kDa, while the observed MW by Western blot was 115kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About US01 / p115
US01 / p115 is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. O60763 NM_003715 NP_003706.2


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Images

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver cancer tissue.
Immunohistochemistry of paraffin-embedded human liver cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human uterine cancer tissue.
Immunohistochemistry of paraffin-embedded human uterine cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil tissue.
Immunohistochemistry of paraffin-embedded human tonsil tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.
Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver cancer tissue.
Immunohistochemistry of paraffin-embedded human liver cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human uterine cancer tissue.
Immunohistochemistry of paraffin-embedded human uterine cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil tissue.
Immunohistochemistry of paraffin-embedded human tonsil tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.
Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver cancer tissue.
Immunohistochemistry of paraffin-embedded human liver cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human uterine cancer tissue.
Immunohistochemistry of paraffin-embedded human uterine cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil tissue.
Immunohistochemistry of paraffin-embedded human tonsil tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.
Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human liver cancer tissue.
Immunohistochemistry of paraffin-embedded human liver cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human uterine cancer tissue.
Immunohistochemistry of paraffin-embedded human uterine cancer tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human tonsil tissue.
Immunohistochemistry of paraffin-embedded human tonsil tissue.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.
Immunohistochemistry of paraffin-embedded human ovarian cancer tissue.

Requested From: United States
Date Requested: 11/13/2018

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