Human UNG / Uracil DNA Glycosylase

Rabbit

Human (tested or 100% immunogen sequence identity)

Monkey, Mouse, Bat, Bovine, Dog, Horse, Rabbit, Chicken (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein G purified

Unmodified

Synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Marmoset, Mouse, Dog, Bat, Bovine, Horse, Rabbit, Turkey, Chicken (94%); Rat, Hamster, Elephant, Panda, Pig, Platypus (89%); Zebrafish (83%).

aa281-298

The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kD, 27.5 kD, 31 kD, and 38 kD forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (2 µg/ml)

PBS, 0.05% Sodium Azide, 0.05% BSA

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13051 NCBI: NM_003362 NP_003353.1