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Catalog Number Size Price
LS-C59547-500 500 µg (5 mg/ml) $606 
ULP1 Antibody - Anti-Yeast ULP-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 ug of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4C with a 1:1000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.
ULP1 Antibody - Anti-Yeast ULP-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4C with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.
ULP1 Antibody - Anti-Yeast ULP-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast ULP-1 antibody was used to confirm the specificity of the antibody. SDS-PAGE of 2 ug of ULP-1 homologues from other sources (lanes 2 through 9). After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4C with a 1:1000 dilution of anti-yULP1 antibody detected as above. This antibody is specific for yeast ULP1 and does not react with ULP1 from related sources including human SENP.
ULP1 Antibody - Anti-Yeast ULP-1 Antibody - Western Blot. Western blot of Affinity Purified anti-Yeast ULP-1 antibody shows detection of a truncated ULP-1 fusion protein (arrowhead). Increasing concentrations of yeast ULP-1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4C with a 1:1000 dilution of anti-yULP-1 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time.
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Polyclonal Rabbit anti‑Yeast ULP1 Antibody (IF, WB) LS‑C59547

Polyclonal Rabbit anti‑Yeast ULP1 Antibody (IF, WB) LS‑C59547

Antibody:
ULP1 Rabbit anti-Yeast Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified
Price
Catalog Number
$606
LS-C59547-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ULP1 Rabbit anti-Yeast Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Yeast
Format:
Unconjugated, Unmodified

Specifications

Description
ULP1 antibody LS-C59547 is an unconjugated rabbit polyclonal antibody to yeast ULP1. Validated for ELISA, IF and WB.
Target
Yeast ULP1
Synonyms
ULP1 | NIB1 | Ulp1p
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined.
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)
Usage
This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kD in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ULP1
Q02724 Q02724

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024