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Catalog Number Size Price
LS-B3043-0.1 0.1 ml $460 
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
UCHL1 / PGP9.5 Antibody - Shows rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.
UCHL1 / PGP9.5 Antibody - Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase, GAPDH (left lane), and UCHL1 / PGP9.5 Antibody (right lane). The GAPDH antibody stains a ~37kD band while the UCHL1 antibody stains a band at about 24kD.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
UCHL1 / PGP9.5 Antibody - Shows rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.
UCHL1 / PGP9.5 Antibody - Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase, GAPDH (left lane), and UCHL1 / PGP9.5 Antibody (right lane). The GAPDH antibody stains a ~37kD band while the UCHL1 antibody stains a band at about 24kD.
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IHC‑plus™ Polyclonal Chicken anti‑Human UCHL1 / PGP9.5 Antibody (IHC, IF, WB) LS‑B3043

IHC‑plus™ Polyclonal Chicken anti‑Human UCHL1 / PGP9.5 Antibody (IHC, IF, WB) LS‑B3043

Note: This antibody replaces LS-C49161
Antibody:
UCHL1 / PGP9.5 Chicken anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3043-0.1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
UCHL1 / PGP9.5 Chicken anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PGP9.5 antibody LS-B3043 is an unconjugated chicken polyclonal antibody to PGP9.5 (UCHL1) from human. It is reactive with human and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human UCHL1 / PGP9.5
Synonyms
UCHL1 | Ubiquitin C-terminal hydrolase | PARK5 | PGP9.5 | PGP95 | Ubiquitin thioesterase L1 | Neuron cytoplasmic protein 9.5 | PGP 9.5 | Uch-L1 | Ubiquitin carboxyl-terminal esterase L1 | HEL-117 | NDGOA | HEL-S-53 | SPG79
Host
Chicken
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgY Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant full length human UCHL1 purified from E. coli
Specificity
UCHL1 - Peripheral Nerve Fiber Marker.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3043 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3043 was determined to be 1:100.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UCHL1 / PGP9.5
P09936 NM_004181 NP_004172.2

Validation

UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

IHC-plus™ UCHL1 / PGP9.5 Antibody for IHC, IF/Immunofluorescence, WB/Western LS-B3043 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024