Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C744972-25 25 µl (5 mg/ml) $275 
Ubiquitin Antibody - Western blot of Ubiquitin. Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche). Other detection systems will yield similar results.

Polyclonal Rabbit anti‑All species Ubiquitin Antibody (Liquid, IHC, WB) LS‑C744972

Polyclonal Rabbit anti‑All species Ubiquitin Antibody (Liquid, IHC, WB) LS‑C744972

Antibody:
Ubiquitin Rabbit Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
All species
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C744972-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Ubiquitin Rabbit Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
All species
Format:
Unconjugated, Liquid

Specifications

Description
Ubiquitin antibody LS-C744972 is an unconjugated rabbit polyclonal antibody to all species Ubiquitin. Validated for ELISA, IHC and WB.
Target
All species Ubiquitin
Host
Rabbit
Reactivity
All species
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
  • IHC
  • Western blot (1:200 - 1:1000)
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021