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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C744972-25 25 µl (5 mg/ml) $235 
Ubiquitin Antibody - Western blot of Ubiquitin. Anti-Ubiquitin antibody, generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche). Other detection systems will yield similar results.

Ubiquitin Antibody LS‑C744972

Ubiquitin Antibody LS‑C744972

Antibody:
Rabbit Polyclonal to All species Ubiquitin
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C744972-25
25 µl
5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to All species Ubiquitin
Application:
WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
Ubiquitin antibody LS-C744972 is an unconjugated rabbit polyclonal antibody to Ubiquitin. Validated for ELISA and WB.
Target
All species Ubiquitin
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:1000)
  • ELISA (1:1000 - 1:5000)
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Ubiquitin
AI525605

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Requested From: United States
Date Requested: 10/23/2019