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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UBE2N / UBC13 Antibody (aa2‑19) LS‑C838
Note: This antibody replaces LS-C93227
UBC13 antibody LS-C838 is an unconjugated rabbit polyclonal antibody to human UBC13 (UBE2N). Validated for IP and WB.
Catalog
Size
Price
LS-C838-100
100 µg (0.5 mg/ml)
$335

Popular UBE2N / UBC13 Products

Anti-UBE2N antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B433 concentration 2.5 ug/ml.
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Anti-UBE2N antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1433 concentration 5 ug/ml.
Species: Human, Mouse, Rat
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Immunohistochemistry image at a dilution of 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, ELISA

Product Description

UBC13 antibody LS-C838 is an unconjugated rabbit polyclonal antibody to human UBC13 (UBE2N). Validated for IP and WB.

Specifications

Target
Human UBE2N / UBC13
Synonyms
UBE2N, BLU, UbcH13, UbcH-ben, Ubiquitin carrier protein N, Yeast UBC13 homolog, UBC13, Ubiquitin-protein ligase N
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (2 µg/ml)
  • Immunoprecipitation
Failed Applications
  • IHC - Paraffin
Immunogen
UBE2N / UBC13 antibody was raised against synthetic peptide from human UBE2N.
Epitope
aa2-19
Specificity
UBE2N.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About UBE2N / UBC13
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. P61088 NM_003348 NP_003339.1


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Images

Western blot

Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.
Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.

Western blot

Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.
Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.

Western blot

Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.
Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.

Western blot

Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.
Detection of UBE2N in human heart lysate with anti-UBE2N pcAb at 2 ug/ml.

Requested From: United States
Date Requested: 10/19/2018

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