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Antibodies > UBE1L / UBA7 > LS-C800407
 
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Catalog Number Size Price
LS-C800407-100 100 µl (1 mg/ml) $379 
LS-C800407-200 200 µl (1 mg/ml) $421 
UBE1L / UBA7 Antibody - 1:100 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
UBE1L / UBA7 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
UBE1L / UBA7 Antibody - Western blot analysis of extracts of HepG2 cells using UBE1L antibody. Lane 1 was treated with the blocking peptide.
UBE1L / UBA7 Antibody - Western blot analysis on HeLa cell lysates using UBE1L antibody. The lane on the left is treated with the antigen-specific peptide.
UBE1L / UBA7 Antibody - 1:100 staining human kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
UBE1L / UBA7 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
UBE1L / UBA7 Antibody - Western blot analysis of extracts of HepG2 cells using UBE1L antibody. Lane 1 was treated with the blocking peptide.
UBE1L / UBA7 Antibody - Western blot analysis on HeLa cell lysates using UBE1L antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human UBE1L / UBA7 Antibody (IHC, IF, WB) LS‑C800407

Polyclonal Rabbit anti‑Human UBE1L / UBA7 Antibody (IHC, IF, WB) LS‑C800407

Submit Review LSBio Guarantee
Antibody:
UBE1L / UBA7 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800407-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
UBE1L / UBA7 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
UBA7 antibody LS-C800407 is an unconjugated rabbit polyclonal antibody to human UBA7 (UBE1L). Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human UBE1L / UBA7
Synonyms
UBA7 | D8 | UBA1B | UBE1L | Ubiquitin-activating enzyme 7 | UBE2 | Ubiquitin-activating enzyme-2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human UBE1L
Specificity
UBE1L antibody detects endogenous levels of total UBE1L.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBE1L / UBA7
P41226 NM_003335 NP_003326.2

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Featured Products

UBE1L / UBA7 Antibody - Immunohistochemical analysis of UBA7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
UBE1L / UBA7 Antibody (C‑Terminus) LS‑C352968
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
UBE1L / UBA7 Antibody - Western blot of recombinant UBE1L / UBA7.
UBE1L / UBA7 Antibody (aa23‑238, PE) LS‑C722935
Species: Mouse
Applications: Western blot
UBE1L / UBA7 Antibody - Western blot of recombinant UBE1L / UBA7.
UBE1L / UBA7 Antibody (aa23‑238) LS‑C314681
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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