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| Catalog Number | Size | Price |
|---|---|---|
| LS-C18912-500 | 500 µg | $606 |
![UBA1 / UBE1 Antibody - Anti-Ubiquitin Activating Enzyme (E1) Antibody - Western Blot. Western blot of purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kD corres-ponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX10 ( for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/uba1-ube1-antibody-ls-c18912/62688_11631.jpg)
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Polyclonal Rabbit anti‑Human UBA1 / UBE1 Antibody (IHC, WB) LS‑C18912
Polyclonal Rabbit anti‑Human UBA1 / UBE1 Antibody (IHC, WB) LS‑C18912
Antibody:
UBA1 / UBE1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
UBA1 / UBE1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
UBE1 antibody LS-C18912 is an unconjugated rabbit polyclonal antibody to human UBE1 (UBA1). Validated for ELISA, IHC and WB.
Target
Human UBA1 / UBE1
Synonyms
UBA1 | A1S9 | A1ST | A1S9T | AMCX1 | GXP1 | Protein A1S9 | SMAX2 | Ubiquitin-activating enzyme E1 | UBE1X | POC20 | UBE1 | UBA1A
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Applications
- IHC
- IHC - Paraffin (2 - 20 µg/ml)
- Western blot (1:1000 - 1:5000)
- ELISA (1:2000 - 1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kD in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. Reconstitute with 0.1 ml of deionized water or equivalent. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About UBA1 / UBE1
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Featured Products
Reactivity:
Mouse
Range:
31.25-2000 pg/ml
Species:
Human, Monkey, Mouse, Rat, Dog
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
