Tyrosinase antibody LS-C18404 is an unconjugated mouse monoclonal antibody to Tyrosinase from human, cat and dog. Validated for ICC, IF, IHC and WB.
Human, Cat, Dog
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Paraffin
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
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Tyrosinase antibody was raised against recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.
Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.
Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistology: 2-4 ug/ml for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4. No preservatives or stabilizing proteins added.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.