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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Tyrosinase Antibody (Azide-free) LS-C18404

Tyrosinase antibody LS-C18404 is an unconjugated mouse monoclonal antibody to Tyrosinase from human, cat and dog. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C18404-100
100 µg
$710
Description
Tyrosinase antibody LS-C18404 is an unconjugated mouse monoclonal antibody to Tyrosinase from human, cat and dog. Validated for ICC, IF, IHC and WB.
Target
Human Tyrosinase
Host
Mouse
Reactivity
Human, Cat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Tyrosinase antibody was raised against recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.
Specificity
Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.
Usage
Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistology: 2-4 ug/ml for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4. No preservatives or stabilizing proteins added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About Tyrosinase
P14679 NM_000372 NP_000363.1

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Requested From: United States
Date Requested: 4/22/2019
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