Tyrosinase antibody LS-C18401 is an unconjugated mouse monoclonal antibody to human Tyrosinase. Validated for IHC, IP and WB.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
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including antigen retrieval solutions, blocking agents
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Tyrosinase antibody was raised against purified recombinant Tyrosinase corresponding to residues 5-456 of human Tyrosinase.
Recognizes Tyrosinase, Mr 70-80kD. An unknown band at ~55kD may also be detected in SKMEL- 19 cell lysates. Species cross-reactivity: Human. Predicted to cross-react with many species based on extensive sequence homology.
Suitable for use in Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Tyrosinase in RIPA lysates from SK-MEL-19 cells. Immunohistochemistry: Detects melanocytic lesions in paraffin embedded tissues.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.