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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TUBB1 / Tubulin Beta 1 Antibody (clone 4A3) IHC‑plus™ LS‑B15615

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
IHC of paraffin-embedded Human endometrium tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Human Kidney tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
Anti-TUBB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB1.
Western blot of extracts (35ug) from 9 different cell lines by using anti-TUBB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
IHC of paraffin-embedded Human endometrium tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
IHC of paraffin-embedded Human Kidney tissue using anti-TUBB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min), at a dilution of 1:150.
Anti-TUBB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB1.
Western blot of extracts (35ug) from 9 different cell lines by using anti-TUBB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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TUBB1 / Tubulin Beta 1 Antibody (clone 4A3) IHC‑plus™ LS‑B15615

Note: This antibody replaces LS-C338196
Antibody:
Mouse Monoclonal to Human TUBB1 / Tubulin Beta 1
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B15615-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human TUBB1 / Tubulin Beta 1
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Tubulin Beta 1 antibody LS-B15615 is an unconjugated mouse monoclonal antibody to Tubulin Beta 1 (TUBB1) from human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TUBB1 / Tubulin Beta 1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
4A3
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human TUBB1(NP_110400) produced in HEK293T cell.
Specificity
Human TUBB1 / Tubulin Beta 1.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUBB1 / Tubulin Beta 1
Q9H4B7 NM_030773 NP_110400.1

Validation

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
IHC Validated
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.

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Requested From: United States
Date Requested: 7/20/2019
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