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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154296-100 100 µg (0.61 mg/ml) $375 
Antibody

Polyclonal Rabbit anti‑Human Alpha Tubulin Antibody (Biotin, aa425‑451, IHC, IF, WB) LS‑C154296

Polyclonal Rabbit anti‑Human Alpha Tubulin Antibody (Biotin, aa425‑451, IHC, IF, WB) LS‑C154296

Antibody:
Rabbit Polyclonal to Human Alpha Tubulin
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Goat, Sheep, Chicken
Format:
Biotin, Unmodified
Price
Catalog Number
$375 
LS-C154296-100
100 µg
0.61 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human Alpha Tubulin
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Goat, Sheep, Chicken
Format:
Biotin, Unmodified

Specifications

Description
Alpha Tubulin antibody LS-C154296 is a biotin-conjugated rabbit polyclonal antibody to Alpha Tubulin (aa425-451) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Human Alpha Tubulin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Goat, Sheep, Chicken (tested or 100% immunogen sequence identity)
Predicted
Fish, Algae, Avian, Bacteria, Mammal, Protozoa (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
Epitope
aa425-451
Specificity
A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Usage
Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 6/5/2020