Tubby antibody LS-C18213 is an unconjugated rabbit polyclonal antibody to human Tubby (TUB). Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
TUB / Tubby antibody was raised against 19 aa peptide sequence near N-terminus of human Tub homolog (31-48 aa) (1). Peptide was coupled to KLH.
The human peptide has no significant homology with mouse tubby, Agouti, AGRP, TULP1 and TULP2. Antibody cross-reactivity in various species is not known. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. ELISA: 1:10000-1:50000 using 50-100 ng control peptide/well.
Lyophilized from PBS, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.