Tubby antibody LS-C18211 is an unconjugated rabbit polyclonal antibody to Tubby (TUB) from human and mouse. Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
TUB / Tubby antibody was raised against a 19 aa peptide sequence near N-terminus of mouse Tubby (505 aa; 2-20 aa) (1). Peptide was coupled to KLH.
The mouse peptide is 100% homologous with human tubby (506 aa). No homology is seen with Agouti, AGRP, TULP1 and TULP2. Antibody cross-reactivity in various species is not known. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. ELISA: 1:10000-1:50000 using 50-100 ng control peptide/well.
0.05% Sodium Azide
100 µl Reconstitute with 0.1ml sterile PBS
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.