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TSLP Antibody LS-C209274

TSLP antibody LS-C209274 is an unconjugated rabbit polyclonal antibody to human TSLP. Validated for ELISA and WB.
Catalog
Size
Price
LS-C209274-100
100 µg (1 mg/ml)
$425
Description
TSLP antibody LS-C209274 is an unconjugated rabbit polyclonal antibody to human TSLP. Validated for ELISA and WB.
Target
Human TSLP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:2000)
  • ELISA (1:20000 - 1:50000)
Immunogen
TSLP antibody was raised against anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
Specificity
This antibody is specific for human TSLP protein. A BLAST analysis was used to suggest cross-reactivity with TSLP from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with TSLP from other sources has not been determined.
Usage
TSLP purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 18 kD in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, sterile filtered
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only.
About TSLP
Q969D9 NM_033035 NP_149024.1

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Images

Western blot

Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.
Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.

Western blot

Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.
Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.

Western blot

Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.
Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.

Western blot

Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.
Western Blot of Rabbit anti-TSLP antibody. Lane 1: Human TSLP Recombinant Protein. Lane 2: None. Load: 50 ng per lane. Primary antibody: TSLP antibody at 1:1,000 for overnight at 4 degrees C. Secondary antibody: DyLight alpha 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 13 kDa, 13 kDa for Human TSLP. Other band(s): None.

Requested From: United States
Date Requested: 5/20/2019
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