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Catalog Number Size Price
LS-C745170-100 100 µg (1 mg/ml) $485 
TS / Thymidylate Synthase Antibody - Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.
TS / Thymidylate Synthase Antibody - Western Blot results of Sheep anti-Thymidylate Synthase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: HeLa WCL Lane 3: MOLT-4 WCL Load: 10µg. Primary Antibody: Sheep anti-Thymidylate Synthase at 1µg/mL overnight at 4°C. Secondary Antibody: Donkey anti-Sheep HRP at 1:40,000 for 30min at RT. Blocking: BlockOut for 30 min at RT.
TS / Thymidylate Synthase Antibody - Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.
TS / Thymidylate Synthase Antibody - Western Blot results of Sheep anti-Thymidylate Synthase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder Lane 2: HeLa WCL Lane 3: MOLT-4 WCL Load: 10µg. Primary Antibody: Sheep anti-Thymidylate Synthase at 1µg/mL overnight at 4°C. Secondary Antibody: Donkey anti-Sheep HRP at 1:40,000 for 30min at RT. Blocking: BlockOut for 30 min at RT.
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Polyclonal Sheep anti‑Human TS / Thymidylate Synthase Antibody (Lyophilized, IHC, IF, WB) LS‑C745170

Polyclonal Sheep anti‑Human TS / Thymidylate Synthase Antibody (Lyophilized, IHC, IF, WB) LS‑C745170

Antibody:
TS / Thymidylate Synthase Sheep anti-Human Polyclonal (Lyophilized) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$485
LS-C745170-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TS / Thymidylate Synthase Sheep anti-Human Polyclonal (Lyophilized) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized

Specifications

Description
Thymidylate Synthase antibody LS-C745170 is an unconjugated sheep polyclonal antibody to human Thymidylate Synthase (TS). Validated for ELISA, IF, IHC and WB.
Target
Human TS / Thymidylate Synthase
Synonyms
TYMS | HST422 | Thymidylate synthetase | TMS | TS | Thymidylate synthase | Tsase
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Lyophilized
Immunogen
Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
Specificity
This antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TS / Thymidylate Synthase
P04818 NM_001071 NP_001062.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2021