(tested or 100% immunogen sequence identity)
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Trypsinogen [Bovine Pancreas].
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas]. Cross reactivity against Trypsinogen from other tissues and species may occur but have not been specifically determined.
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:40000)
Anti-TRYPSINOGEN (RABBIT) Antibody has been assayed against 1.0 ug of Trypsinogen [Bovine Pancreas] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:3000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitute in 100 µL of deionized water.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.