(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
Trypsinogen [Bovine Pancreas].
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas]. Cross reactivity against Trypsinogen from other sources may occur but has not been specifically determined.
Anti-Trypsinogen antibody has been assayed against 1.0 ug of Trypsinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:20000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA
Reconstitute with distilled water.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
For research use only.
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