(tested or 100% immunogen sequence identity)
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Western blot (1:500 - 1:3000)
- ELISA (1:5000 - 1:25000)
- Dot Blot
Trypsinogen [Bovine Pancreas].
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas]. Cross reactivity against Trypsinogen from other sources may occur but has not been specifically determined.
Anti-Trypsinogen antibody has been assayed against 1.0 ug of Trypsinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:20000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitute in 100 µL of deionized water.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
For research use only. Intended for use by laboratory professionals.
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