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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRPV4 Antibody (Internal) IHC‑plus™ LS‑A8583
TRPV4 antibody LS-A8583 is an unconjugated rabbit polyclonal antibody to TRPV4 from human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Catalog
Size
Price
LS-A8583-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular TRPV4 Products

Antibody
Species: Mouse, Human, Rat
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot
Antibody
Species: Mouse, Human, Rat
Applications: IHC, Western blot
Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Mouse, Human, Rat
Applications: IHC, Western blot

Product Description

TRPV4 antibody LS-A8583 is an unconjugated rabbit polyclonal antibody to TRPV4 from human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.

Specifications

Target
Human TRPV4
Synonyms
TRPV4, CMT2C, HMSN2C, OTRPC4, SSQTL1, VR-OAC, VRL-2, Osm-9-like TRP channel 4, SPSMA, TRP12, VRL2, VROAC, SMAL
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Rabbit, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TRPV4 antibody was raised against synthetic 20 amino acid peptide from internal region of human TRPV4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Horse, Pig (100%); Elephant, Rabbit (95%); Turkey, Chicken, Platypus (90%); Xenopus (85%); Stickleback, Zebrafish (80%).
Epitope
Internal
Specificity
Human TRPV4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TRPV4
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Q9HBA0 NM_021625 NP_067638.3


Publications (3)

1
The polymodal ion channel transient receptor potential vanilloid 4 modulates calcium flux, spiking rate, and apoptosis of mouse retinal ganglion cells. Ryskamp DA, Witkovsky P, Barabas P, Huang W, Koehler C, Akimov NP, Lee SH, Chauhan S, Xing W, Rentera RC, Liedtke W, Krizaj D. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:7089-101. (WB, IHC-Fr; Mouse, Human) [Full Text Article] [PubMed:21562271] [PMC:PMC3109951]
2
Activation of endothelial TRPV4 channels mediates flow-induced dilation in human coronary arterioles: role of Ca2+ entry and mitochondrial ROS signaling. Bubolz AH, Mendoza SA, Zheng X, Zinkevich NS, Li R, Gutterman DD, Zhang DX. American journal of physiology. Heart and circulatory physiology. 2012 Feb;302:H634-42. [Full Text Article] [PubMed:22140047] [PMC:PMC3353785]
3
Pregnancy-induced remodelling and enhanced endothelium-derived hyperpolarization-type vasodilator activity in rat uterine radial artery: transient receptor potential vanilloid type 4 channels, caveolae and myoendothelial gap junctions. Senadheera S, Bertrand PP, Grayson TH, Leader L, Murphy TV, Sandow SL. Journal of anatomy. 2013 Dec;223:677-86. [Full Text Article] [PubMed:24128141]

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Images

Immunohistochemistry

Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRPV4 antibody LS-A8583 IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/17/2018

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