TRPC6 antibody LS-B14655 is an unconjugated goat polyclonal antibody to TRPC6 from human and mouse. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
TRPC6 antibody was raised against synthetic peptide C-KQSSTRSSEDYHLNS from an internal region of mouse TRPC6 (NP_038866.2). Percent identity by BLAST analysis: Mouse (100%); Rat (93%); Elephant, Porcine, Opossum, Guinea pig, Platypus (87%); Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Panda, Dog, Bovine, Horse, Rabbit (80%).
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 75kD band in Mouse and Rat Brain and Spinal Cord lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kD according to NP_038866.2. The 75kD band was successfully blocked by incubation with the immunizing peptide.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Store at -20°C. Avoid freeze-thaw cycles.