(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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TRPC6 antibody was raised against peptide with sequence C-KQSSTRSSEDYHLNS, from the internal region of the protein sequence according to NP_038866.2.
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B14655. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 75kD band in Mouse and Rat Brain and Spinal Cord lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kD according to NP_038866.2. The 75kD band was successfully blocked by incubation with the immunizing peptide.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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