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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B177-100 100 µl (1 mg/ml) $425 
TRPA1 Antibody - TRPA1 antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cerebellum, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ TRPA1 Antibody aa1‑100 Polyclonal IHC LS‑B177

IHCPlus™ TRPA1 Antibody aa1‑100 Polyclonal IHC LS‑B177

Note: This antibody replaces LS-C19333
Antibody:
Rabbit Polyclonal to Human TRPA1
Application:
IHC, IHC-P, IHC-Fr
Reactivity:
Human, Mouse, Guinea pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B177-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TRPA1
Application:
IHC, IHC-P, IHC-Fr
Reactivity:
Human, Mouse, Guinea pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
TRPA1 antibody LS-B177 is an unconjugated rabbit polyclonal antibody to TRPA1 from human, mouse and guinea pig. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human TRPA1
Host
Rabbit
Reactivity
Human, Mouse, Guinea pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide made to a region within the N-Terminus (residues 1-100) of the human TRPA1 protein. [Swiss-Prot# O75762]
Epitope
aa1-100
Specificity
TRPA1.
Usage
Immunohistochemistry: LS-B177 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B177 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRPA1
O75762 NM_007332 CAA71610.1

Validation

TRPA1 Antibody - TRPA1 antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cerebellum, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRPA1 antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cerebellum, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.
TRPA1 antibody (5 ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cerebellum, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 12/8/2019