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Catalog Number Size Price
LS-C154244-100 100 µg (0.67 mg/ml) $567 
TRIM29 Antibody - Anti-ATDC (Ac-K116) Antibody - Western Blot. Western blot of affinity purified anti-ATDC (Ac-K116) antibody shows detection of a 66 kD band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation. Personal communication, Z. Yuan, H Lee Moffitt Cancer Center and Research Institute.

Polyclonal Rabbit anti‑Human TRIM29 Antibody (Lys116, Internal, WB) LS‑C154244

Polyclonal Rabbit anti‑Human TRIM29 Antibody (Lys116, Internal, WB) LS‑C154244

Antibody:
TRIM29 Rabbit anti-Human Polyclonal (Lys116, Internal) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Bovine, Horse, Macaque
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154244-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TRIM29 Rabbit anti-Human Polyclonal (Lys116, Internal) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Bovine, Horse, Macaque
Format:
Unconjugated, Unmodified

Specifications

Description
TRIM29 antibody LS-C154244 is an unconjugated rabbit polyclonal antibody to TRIM29 (Lys116, Internal) from human. It is reactive with human, bovine, chimpanzee and other species. Validated for ELISA and WB. Cited in 2 publications.
Target
Human TRIM29
Synonyms
TRIM29 | ATDC | Tripartite motif containing 29 | Tripartite motif-containing 29
Host
Rabbit
Reactivity
Human, Chimpanzee, Bovine, Horse, Macaque (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Bovine, Horse, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
Epitope
Lys116, Internal
Specificity
This antibody reacts with over-expressed, acetylated lysine ATDC protein. A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, cattle, chimpanzee and macaque based on a 100% homology with the immunizing sequence. Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence. Cross-reactivity with ATDC from other sources has not been determined.
Applications
  • Western blot (1:500 - 1:3000)
  • ELISA (1:100000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity and detection of ATDC Ac-K116 should be optimized by the end user. Expect a band approximately ~66 kD in size corresponding to ATDC Ac-K116 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRIM29
Q14134 NM_012101 NP_036233.2

LSBio Ratings

TRIM29 Antibody (Lys116, Internal) for WB/Western, ELISA LS-C154244 has an LSBio Rating of
Publications (4.1)

Publications (2)

The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions. Yuan Z, Villagra A, Peng L, Coppola D, Glozak M, Sotomayor EM, Chen J, Lane WS, Seto E. Molecular and cellular biology. 2010 30:3004-15.

Histone deacetylase 9 (HDAC9) regulates the functions of the ATDC (TRIM29) protein. Yuan Z, Peng L, Radhakrishnan R, Seto E. The Journal of biological chemistry. 2010 285:39329-38.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024