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Catalog Number	Size	Price
LS-C287177-10	10 µl	$295
LS-C287177-100	100 µl (1 mg/ml)	$514

TRIM28 / KAP1 Antibody - Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:1000 (1μg/ml). Detection: DAB (left) and green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).

TRIM28 / KAP1 Antibody - Detection of mouse and rat KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

TRIM28 / KAP1 Antibody - Detection of human KAP-1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and HEK293T (50µg) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

TRIM28 / KAP1 Antibody - Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

TRIM28 / KAP1 Antibody - Detection of Mouse KAP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa1‑50, IHC, WB) LS‑C287177

Name: Anti-TRIM28 / KAP1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C287177-10
Price: 295.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa1-50) Antibody

Application:

IHC, ICC, WB, IP

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

$295

LS-C287177-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa1-50) Antibody

Application:

IHC, ICC, WB, IP

Reactivity:

Human, Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

KAP1 antibody LS-C287177 is an unconjugated rabbit polyclonal antibody to KAP1 (TRIM28) (aa1-50) from human. It is reactive with human and mouse. Validated for ICC, IHC, IP and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human TRIM28 / KAP1

Synonyms

See All TRIM28 / KAP1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purified

Modifications

Unmodified

Immunogen

A portion of GeneID: 10155 corresponding to amino acids between 1 and 50 of UniprotID: Q13263

Epitope

aa1-50

Specificity

Region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).

Applications

IHC (1:500 - 1:2000)
ICC (1:500 - 1:2500)
Western blot (1:2000 - 1:10000)
Immunoprecipitation (2 - 10 µg/mg lysate)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Manufacturer

Bethyl Laboratories, Inc.

Presentation

Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.

Storage

Store at 2-8°C for up to one year.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TRIM28 / KAP1

Uniprot: Q13263 NCBI: NM_005762 NP_005753.1

LSBio Ratings

TRIM28 / KAP1 Antibody (aa1-50) for IHC, ICC, WB/Western, IP LS-C287177 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Absolute Quantification of Transcription Factors Reveals Principles of Gene Regulation in Erythropoiesis. Mark A Gillespie, Carmen G Palii, Daniel Sanchez-Taltavull, Paul Shannon, William J R Longabaugh, Damien J Downes, Karthi Sivaraman, Herbert M Espinoza, Jim R Hughes, Nathan D Price, Theodore J Perkins, Jeffrey A Ranish, Marjorie Brand. Molecular cell. 2020 June;78:960-974.e11.

Full Text Article PubMed: 32330456 PMC: PMC7344268

Submit a Publication for LS-C287177