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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TRAF3 Antibody LS‑C679883
TRAF3 antibody LS-C679883 is an unconjugated rabbit polyclonal antibody to human TRAF3. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C679883-50
50 µg
$285
LS-C679883-100
100 µg
$385
Description
TRAF3 antibody LS-C679883 is an unconjugated rabbit polyclonal antibody to human TRAF3. Validated for ELISA, IF and WB.
Target
Human TRAF3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
TRAF3 antibody was raised against recombinant Human TNF receptor-associated factor 3 protein (1-40AA)
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
About TRAF3
Q13114 NM_145725 NP_663777.1

Popular TRAF3 Products

Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-TRAF3 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-TRAF3 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Western blot of TRAF3 in various tumor cell lines using Polyclonal Antibody to TRAF3 at 1:2000. TRAF3 is observed at ~67 kD.
Species: Human, Mouse, Rat, Gerbil
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Pig, Sheep
Applications: ICC, Immunofluorescence, Western blot

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Images

Immunofluorescence

Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa
Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa

Immunofluorescence

Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa
Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa

Immunofluorescence

Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa
Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa

Immunofluorescence

Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa
Western Blot Positive WB detected in:HEK293 whole cell lysate All Lanes:TRAF3 antibody at 3.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65,56 KDa Observed band size: 65 KDa

Requested From: United States
Date Requested: 12/11/2018
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