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Antibodies > TRAF1 > LS-C313135
 
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Catalog Number Size Price
LS-C313135-10 10 µg $318 
LS-C313135-100 100 µg (0.5 mg/ml) $470 
TRAF1 Antibody - Western blot analysis of TRAF1 using anti-TRAF1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
TRAF1 Antibody - WB of TRAF1 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human TRAF1, 30.6KD. (162aa tag+ E174-E282). . Lane1: Recombinant Human TRAF1 Protein 5ng. Lane2: Recombinant Human TRAF1 Protein 2.5ng. Lane3: Recombinant Human TRAF1 Protein 1.25ng..
TRAF1 Antibody - Western blot analysis of TRAF1 using anti-TRAF1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD.
TRAF1 Antibody - WB of TRAF1 antibody. Recombinant Protein Detection Source:. E.coli derived -recombinant Human TRAF1, 30.6KD. (162aa tag+ E174-E282). . Lane1: Recombinant Human TRAF1 Protein 5ng. Lane2: Recombinant Human TRAF1 Protein 2.5ng. Lane3: Recombinant Human TRAF1 Protein 1.25ng..
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Polyclonal Rabbit anti‑Human TRAF1 Antibody (aa220‑235, WB) LS‑C313135

Polyclonal Rabbit anti‑Human TRAF1 Antibody (aa220‑235, WB) LS‑C313135

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Note: This antibody replaces LS-C389321
Antibody:
TRAF1 Rabbit anti-Human Polyclonal (aa220-235) Antibody
Application:
WB
Reactivity:
Human, Monkey, Guinea pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313135-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TRAF1 Rabbit anti-Human Polyclonal (aa220-235) Antibody
Application:
WB
Reactivity:
Human, Monkey, Guinea pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
TRAF1 antibody LS-C313135 is an unconjugated rabbit polyclonal antibody to TRAF1 (aa220-235) from human. It is reactive with human, guinea pig, monkey and other species. Validated for WB.
Target
Human TRAF1
Synonyms
TRAF1 | EBI6 | MGC:10353
Host
Rabbit
Reactivity
Human, Monkey, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TRAF1(220-235 aa HQSQLDRERILSLEQR), different from the related mouse sequence by one amino acid.
Epitope
aa220-235
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRAF1
Q13077 NM_005658 NP_005649.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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