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Catalog Number Size Price
LS-C95183-100 100 µl $393 
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer).
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer).
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer).
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). WB on rat brain and spinal cord lysate using Rabbit antibody to TPC1 (750-800), Dilution: 1:500, incubated overnight at 4C in MPX from LiCor. Blocking buffer: 0.2 micron filtered 0.5% LFDM in 1x PBS containing 0.01% Tween-20. Washing buffer: 1x PBS. Detection: ECL using C-Digit from LiCor.
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer).
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer).
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). IHC-P on paraffin sections of rat kidney using Rabbit antibody to TPC1 (750-800) HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer).
TPC1 / TPCN1 Antibody - Rabbit antibody to TPC1 (750-800). WB on rat brain and spinal cord lysate using Rabbit antibody to TPC1 (750-800), Dilution: 1:500, incubated overnight at 4C in MPX from LiCor. Blocking buffer: 0.2 micron filtered 0.5% LFDM in 1x PBS containing 0.01% Tween-20. Washing buffer: 1x PBS. Detection: ECL using C-Digit from LiCor.
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Polyclonal Rabbit anti‑Mouse TPC1 / TPCN1 Antibody (aa750‑800, IHC, WB) LS‑C95183

Polyclonal Rabbit anti‑Mouse TPC1 / TPCN1 Antibody (aa750‑800, IHC, WB) LS‑C95183

Antibody:
TPC1 / TPCN1 Rabbit anti-Mouse Polyclonal (aa750-800) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C95183-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TPC1 / TPCN1 Rabbit anti-Mouse Polyclonal (aa750-800) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TPCN1 antibody LS-C95183 is an unconjugated rabbit polyclonal antibody to TPCN1 (TPC1) (aa750-800) from mouse. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Mouse TPC1 / TPCN1
Synonyms
TPCN1 | KIAA1169 | Two-pore segment channel 1 | TPC1 | Two pore segment channel 1 | Two-pore channel 1
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
Synthetic peptide from the C-Terminus of mouse TPC1 (Two pore calcium channel protein 1) conjugated to an immunogenic carrier protein. The antigen is homologous in rat and shares 98% identity with human sequence.
Epitope
aa750-800
Specificity
Specific for TPC1.
Applications
  • IHC
  • IHC - Paraffin (1:300 - 1:2000)
  • IHC - Frozen (1:300 - 1:2000)
  • Western blot (1:300 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval is essential for use on paraffin sections.
Presentation
Lyophilized
Reconstitution
100 µl Sterile water
Storage
Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TPC1 / TPCN1
Q9ULQ1 AX235888 CAC88241.1

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Requested From: United States
Date Requested: 4/25/2024