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Catalog Number Size Price
LS-C750569-20 20 µl $253 
LS-C750569-50 50 µl $306 
LS-C750569-100 100 µl $381 
LS-C750569-200 200 µl $489 
TP53 / p53 Antibody - Western blot analysis of extracts of HT-29 cells, using Phospho-TP53-S33 antibody at 1:2000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.
TP53 / p53 Antibody - Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug Phospho-TP53-S33 pAb. Western blot was performed from the immunoprecipitate using Phospho-TP53-S33 pAb at a dilition of 1:1000. HT-29 cells were treated by nocodazole (100 ng/mL) at 37°C for 16 hours.
TP53 / p53 Antibody - Western blot analysis of extracts of HT-29 cells, using Phospho-TP53-S33 antibody at 1:2000 dilution. HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.
TP53 / p53 Antibody - Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug Phospho-TP53-S33 pAb. Western blot was performed from the immunoprecipitate using Phospho-TP53-S33 pAb at a dilition of 1:1000. HT-29 cells were treated by nocodazole (100 ng/mL) at 37°C for 16 hours.
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Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (phospho‑Ser33, WB) LS‑C750569

Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (phospho‑Ser33, WB) LS‑C750569

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (pSer33) Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C750569-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (pSer33) Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-C750569 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (pSer33). Validated for IP and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer33
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 23-43kDa, while the observed MW by Western blot was 55kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021