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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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TOPBP1 Antibody LS‑C668199 Best Seller
TOPBP1 antibody LS-C668199 is an unconjugated rabbit polyclonal antibody to human TOPBP1. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C668199-50
50 µl (1 mg/ml)
$235
LS-C668199-100
100 µl (1 mg/ml)
$275
Description
TOPBP1 antibody LS-C668199 is an unconjugated rabbit polyclonal antibody to human TOPBP1. Validated for ICC, IF, IHC and WB.
Target
Human TOPBP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • ICC
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
TOPBP1 antibody was raised against recombinant full length protein of human TOPBP1
Specificity
Recognizes endogenous levels of TOPBP1 protein.
Usage
Western blot analysis of TOPBP1 expression in HEK293T (A) whole cell lysates. Immunohistochemical analysis of TOPBP1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of TOPBP1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About TOPBP1
Q92547 NM_007027 NP_008958.2

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Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
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Immunohistochemistry of paraffin-embedded rat lung using TOPBP1 antibody at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at 1:100 dilution.
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Antibody
Species: Human
Applications: ICC, Western blot

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Images

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot


Requested From: United States
Date Requested: 11/17/2018
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