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Antibodies > TOP2B / Topoisomerase II Beta > LS-C800402
 
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Catalog Number Size Price
LS-C800402-100 100 µl (1 mg/ml) $379 
LS-C800402-200 200 µl (1 mg/ml) $421 
TOP2B / Topoisomerase II Beta Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
TOP2B / Topoisomerase II Beta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
TOP2B / Topoisomerase II Beta Antibody - Western blot analysis on Jurkat cell lysates using TOP2B antibody. The lane on the left is treated with the antigen-specific peptide.
TOP2B / Topoisomerase II Beta Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
TOP2B / Topoisomerase II Beta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
TOP2B / Topoisomerase II Beta Antibody - Western blot analysis on Jurkat cell lysates using TOP2B antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human TOP2B / Topoisomerase II Beta Antibody (IHC, IF, WB) LS‑C800402

Polyclonal Rabbit anti‑Human TOP2B / Topoisomerase II Beta Antibody (IHC, IF, WB) LS‑C800402

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Antibody:
TOP2B / Topoisomerase II Beta Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800402-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TOP2B / Topoisomerase II Beta Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Topoisomerase II Beta antibody LS-C800402 is an unconjugated rabbit polyclonal antibody to Topoisomerase II Beta (TOP2B) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human TOP2B / Topoisomerase II Beta
Synonyms
TOP2B | DNA topoisomerase II beta | DNA topoisomerase 2-beta | Topo II beta | Antigen MLAA-44 | TOPIIB | Topoisomerase II beta | Topoisomerase IIb | U937 associated antigen | DNA topoisomerase II, 180 kD | Top2beta
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human TOP2B.
Specificity
TOP2B antibody detects endogenous levels of total TOP2B.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TOP2B / Topoisomerase II Beta
Q02880 NM_001068 NP_001059.2

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Featured Products

TOP2B / Topoisomerase II Beta Antibody - Immunohistochemical analysis of Topoisomerase 2 beta staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TOP2B / Topoisomerase II Beta Antibody (N‑Terminus) LS‑C352951
Species: Human, Monkey, Dog, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
KRAS Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
KRAS Antibody (clone 2C1) LS‑C175665
Species: Human
Applications: IHC - Paraffin, Western blot
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DNASE1 / DNase I ELISA Kit
Mouse DNASE1 / DNase I (Sandwich ELISA) ELISA Kit - LS-F16113
Reactivity: Mouse
Range: 78-5000 pg/ml
Osteoglycin / Mimecan Protein - Recombinant Osteoglycin By SDS-PAGE
Human Osteoglycin / Mimecan Protein (Recombinant His) (aa180-298) - LS-G15022
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Tag: His
Copeptin ELISA Kit
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Reactivity: Human
Range: 7.8-500 pg/ml
See all TOP2B / Topoisomerase II Beta Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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